Research Paper Volume 15, Issue 16 pp 7909—7921

Figure 1. Differential and temporal gene expression signatures in aged pancreas. (A) Pairwise correlation of normalized gene expression (transcripts per million; TPM) for young and old RNA-Seq samples (n=12). Colors indicate the Pearson correlation. (B) Principal Component Analysis (PCA) of temporal gene expression from young and old mice samples (n = 12). (C) Top: Volcano plot showing differential gene expression profile with significantly (p < 0.05, fold change ≥ 1.5) upregulated (n = 133 genes) and downregulated (n = 251) genes in old mice when compared to young. (D) Enriched molecular pathways corresponding to up and downregulated genes. (E) Top: Venn diagram of rhythmic genes in young and old mice. Bottom: Heatmap of z-scored rhythmically expressed genes in young and old mice. Heatmaps are double plotted for time.