Research Paper Volume 15, Issue 16 pp 7866—7908

Figure 6. Dectin-1 stimulation leads to distinct signatures of differentiation. GSEA was performed on normalized counts obtained from Dectin-1 stimulated Inflammatory monocytes using previously defined gene sets for in vitro moDCs and Activated DCs. (A) Box plots of CSF1, CSF2, NR4A1, and NR4A3. The symbol * represents significant differentially expressed genes with fold cutoff of 1.2, and q value < 0.1. (B) Gene enrichment analysis for monocyte differentiation was performed. The plot represents normalized enrichment scores and FDR (q-values) obtained for each cohort. For graphical representation, FDR values with 0 were adjusted to 0.00001 and FDR of <5% was considered significant for the enrichment of a particular term. (C) Transcription factor analysis of the significantly enriched activated DCs gene sets using gProfiler and visualization by Cytoscape. * Significantly differentially expressed genes with FC cut off 1.2 and padj <0.1.