Research Paper Volume 15, Issue 16 pp 7866—7908

Figure 2. Dectin-1 surface expression in monocytes and dendritic cells. The cohort consists of the following: Monocytes: [HIV-negative young adults (Young) (n=21), HIV-negative older adults (Older) (n= 24), HIV-positive young adults (HIV-young) (n =14), and HIV-positive older adults (HIV-Older) (n = 22)]. Dendritic cells: [Young (n=21), Older (n=18), HIV-Young (n=10), HIV-Older (n= 17)]. Dot plots showing the Median of the Mean Fluorescence Intensity (MFI) of Dectin-1 surface expression. The following comparisons indicated by asterisks were statistically significant using a Wilcoxon two-sample test with t approximation, which were then adjusted with a false discover rate (FDR) calculation for multiple comparisons. (A) Activated Monocytes (CD11b+ CD14+). Young vs. Older (p= 0.026), Young vs. HIV-Young (p=0.001), Older vs. HIV-Older, (p=0.005). (B) Inflammatory Monocytes (CD14+CD16+). Young vs. Older (p= 0.003), Young vs. HIV-Young (p=0.001). (C) Classical Monocytes (CD14+CD16lo). Young vs. Older (p= 0.0039), Young vs. HIV-Young (p=0.0026). (D) Non-classical monocytes (CD14lo, CD16H). Young vs. HIV-Young (p=0.011), Older vs. HIV-Older, (p=0.048). (E) Dectin-1 Surface Expression in Myeloid Dendritic cells (mDC) and Plasmacytoid Dendritic Cells (pDC). i). mDC, Young vs. HIV-Young (p=0.0002), Older vs. HIV-Older (p=0.0018). ii). pDC, Young vs. HIV-Young (p=0.012), Older vs. HIV-Older (p=0.0085).