Research Paper Volume 15, Issue 15 pp 7565—7582
Unveiling the prognostic significance of SOX5 in esophageal squamous cell carcinoma: a comprehensive bioinformatic and experimental analysis
Figure 10. Upregulation of SOX5 inhibited proliferation and increased apoptosis of ESCC cells in vitro. (A) Western blot assay of SOX5 and hub genes expression in ESCC and Het1A cells. (B) Western blot assay of SOX5 protein in control and overexpression ESCC cells. (C, D) CCK-8 assay (C) and EdU assay (D) results of different treated cells. (E) Western blot assay of Bcl2, Bax, cleaved-caspase 3 and caspase 3 protein in different treated cells. (F) Flow cytometry analysis of apoptosis of different treated cells. *P < 0.05, **P < 0.01.