Figure 8. The protein concentration of CXCL2 in the extracellular medium as determined using ELISA (A). The impact of three concentrations of human recombinant CXCL2 protein on cell proliferation as determined by the CCK-8 assay (B). The impact on migration and invasion as evaluated using the Transwell assay (C-1, C-2). The mRNA expression of EMT markers was in three different cell culture environments (D). The migration, invasion, and resistance to sunitinib outcomes in three different cell culture environments (E-1–E-3).