Research Paper Volume 15, Issue 15 pp 7533—7550

Figure 1. HBx promoted proliferation, migration and invasion of hepatoma cells. (A) HBx overexpression promoted the expression of PCNA, CDH2, and snail by RT-qPCR detection. (B) HBx elevated the expression of proliferative migration markers by western blotting. (C, D) EdU and CCK-8 assays confirmed the effect of HBx overexpression on the proliferation of Huh-7 cells. (E, F) HBx overexpression also promoted migration and invasion by both wound-healing assays and Transwell. The experiments were repeated at least 3 independent times. (C–F) Data was presented as mean ± SD from three independent experiments. ^*p < 0.05; ^**p < 0.01.