Research Paper Volume 15, Issue 15 pp 7451—7475

SLC1A5 is a novel biomarker associated with ferroptosis and the tumor microenvironment: a pancancer analysis

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Figure 9. SLC1A5 inhibits ferroptosis in glioma cells. (A, B) The level of GSH was measured in U251 and U118 cells after SLC1A5 knockdown. (C, D) The level of MDA was measured in U251 and U118 cells after SLC1A5 knockdown. (E, F) The RNA expression levels of the ferroptosis gene GPX4 were detected in U251 and U118 cell lines after SLC1A5 knockdown. (G, H) The RNA expression levels of the ferroptosis gene ACSL4 in U251 and U118 cell lines were detected after SLC1A5 knockdown. (I) Western blotting was used to detect the protein expression levels of GPX4 and ACSL4 after SLC1A5 knockdown. The labeled asterisk indicates the statistical p value (*p < 0.05, **p < 0.01, ***p < 0.001).