Research Paper Volume 15, Issue 15 pp 7451—7475
SLC1A5 is a novel biomarker associated with ferroptosis and the tumor microenvironment: a pancancer analysis
Figure 8. In vitro evidence that SLC1A5 is involved in the proliferation and migration of glioma cells. (A) RNA and (B) protein expression levels of SLC1A5 in astrocytes and glioma cell lines. (C–E) RT‒qPCR and Western blotting verified the knockdown efficiency of SLC1A5 in U251 and U118 cell lines, respectively. (F) Colony formation experiments were performed to analyze the effect of SLC1A5 downregulation on the colony formation ability of U251 and U118 cell lines, and (G, H) statistical analysis was performed. (I, J) Cell proliferation ability was evaluated using the CCK-8 assay, and proliferation curves were plotted. (K) Cell migration ability was determined by transwell migration assay. The labeled asterisk indicates the statistical p value (*p < 0.05, **p < 0.01, ***p < 0.001).