Research Paper Volume 15, Issue 15 pp 7440—7450

Figure 2. Downregulation of hsa_circ_0010889 suppressed glioma proliferation and tumor growth both in vivo and in vitro. (A) RT-qPCR detection showing the expression of hsa_circ_0010889 in both LN229 and U251 cells. The data are presented as the mean ± SD. ^***p < 0.001 vs. NC. (B, C) CCK8 detection showing the effect of hsa_circ_0010889 on glioma cell proliferation. The data are presented as the mean ± SD. ^***p < 0.001 vs. NC. (D, E) EdU detection showing cell proliferation in both LN229 and U251 cells. Data are presented as the mean ± SD. ^***P < 0.001 vs. si-NC. (F) Representative images of LN229 tumor formation in xenografts from nude mice. (G, H) Summary of tumor volumes and weights in mice. Data are presented as the mean ± SD. ^***P < 0.001 vs. sh-NC. (I, J) Immunohistochemical staining showing the percentage of Ki-67-positive cells and relative Ki-67-positive cells were calculated. Data are presented as the mean ± SD. ^***P < 0.001 vs. sh-NC.