Research Paper Volume 15, Issue 16 pp 7933—7955
A novel hepatocellular carcinoma-specific mTORC1-related signature for anticipating prognosis and immunotherapy
Figure 7. Molecular pathways and experimental verification of the three genes. (A) PI3K-AKT signaling was enhanced in the high-expression group in TCGA-LIHC and ICGC-LIRI-JP datasets. (B) MAPK pathways was significantly correlated with our model. (C) The expression of PAG1, LHFPL2, and FABP5 in high HCC metastatic cell lines were higher than those in HCC non-metastatic cell lines. (D) The expression of PAG1 and LHFPL2 were significantly downregulated in MHCC97H cell lines, whereas the expression of FABP5 was enhanced in MHCC97H cell lines after treating with mTORC1 blocker or MEK/ERK inhibitors.