Research Paper Volume 15, Issue 11 pp 4949—4962

Figure 4. PZR overexpression promotes proliferation, migration, and invasion of SPC-A1 cells. SPC-A1 cells were infected with recombinant lentiviruses carrying PZR (PZR-OE) or the empty vector (V-OE), and stable cells were selected by treatment with puromycin. (A) Western blotting verified overexpression of PZR. (B) PZR-OE SPC-A1 cells showed increased colony-forming ability. (C) Wound healing assays demonstrated accelerated migration of PZR-OE SPC-A1 cells. Images showed the wounded monolayers of V-OE and PZR-OE SPC-A1 cells at 0 h, 24 h and 48 h (magnification, ×40). (D) Transwell invasion assays with Matrigel revealed enhanced penetration ability of PZR-OE SPC-A1 cells (magnification, ×100). All assays were done in triplicates. Data in bar graphs represent mean ± SD (n = 3). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001.