Research Paper Volume 15, Issue 11 pp 4949—4962

Figure 3. PZR-knockout SPC-A1 cells exhibit reduced proliferative, migrating, and invading ability. (A) Colonies formed by wild type and PZR-KO SPC-A1 cells were stained with crystal violet and then numerated. (B) Morphology of wild type and PZR-KO SPC-A1 cell (magnification, ×200). (C) Wound healing assays. Images show the wounded monolayers of wild type and PZR-KO SPC-A1 cells at 0 h, 24 h and 48 h (magnification, ×40). Black lines outline wound edges, and the distance between the lines was measured using the Image J software. The histogram shows migration distance after 24 h and 48 h. (D) Transwell invasion assays. Cells that passed through Matrigel and attached to the Transwell membrane were stained with crystal violet and numerated (magnification, ×100). All assays were done in triplicates. Data in bar graphs represent mean ± SD (n = 3). ^**P < 0.01, ^***P < 0.001.