Figure 7. IRGs regulates the biological behavior of Ishikawa cell lines. (A) PCR was used to verify knockdown efficiency of ROS1. (B, C) CCK-8 and colony formation assays were used to evaluate the proliferation effect of ROS1. (D) Effect of ROS1 on invasion assessed using the Transwell assay. (E) Cell apoptosis assay was used to determine the effect of LAMP3, MEP1A and ROS1.