Research Paper Volume 15, Issue 11 pp 4576—4599
Senescence and senotherapies in biliary atresia and biliary cirrhosis
Figure 2. Cholangiocytes and perinodular hepatocytes display cellular senescence in BA livers. (A) Co-staining of SA-β-gal activity and CK-19 IHC on BA livers: some bile ductules cholangiocytes show staining co-localization (arrowheads) while perinodular hepatocytes are only positive for SA-β-gal (arrows); (B) Serial staining of SA-β-gal activity and CK-19 IHC on BA livers cryosections. Left and middle images: bile ductules (arrowheads) and perinodular hepatocytes (arrows) are positive for SA-β-gal in both early and late stage BA. Right images: remaining large septal bile duct display SA-β-gal activity. BA: biliary atresia; IHC: immunohistochemistry; SA-β-gal: senescence-associated beta-galactosidase. Scale bars = 100 μm.