Research Paper Volume 15, Issue 7 pp 2610—2630
Bioinformatics analysis and experimental validation of a novel autophagy-related signature relevant to immune infiltration for recurrence prediction after curative hepatectomy
Figure 9. Immunohistochemistry and cell experiments of gene SNRPE to validate our results. (A) Compared with adjacent normal liver tissues (a), SNRPE protein expression was significantly overexpressed in HCC tissues (b) (×200 magnification). (B) SNRPE mRNA expression was greater in HCC cells than in the normal liver cells. (C) The shSNRPE with lentiviral transfection was transfected into HepG2 cells. (D) CCK8 assay. After SNRPE knockdown, the HepG2 cells exhibited a significant inhibition in viability. (E, F) Transwell assay. After SNRPE knockdown, the HepG2 cells exhibited a significant inhibition in migration and invasion.