Research Paper Volume 15, Issue 7 pp 2525—2540

Figure 5. DDX5 promotes gastric cancer cells growth and migration by regulating circPHF14. (A) AGS or 7901 cells transfected with control siRNA or siRNA specifically targeting circPHF14 (si-circPHF14) in the presence DDX5 were subjected to colony formation assay. (B) CCK-8 assay of proliferation in AGS and 7901 cells following a time course of transfection with NC or DDX5 or DDX5 and si-circPHF14. (C) EdU assay was conducted to test the effect of DDX5 and circPHF14 on the proliferation of AGS and 7901 cells. Scale bar 100μm. (D) A migration rescue experiment verified the effect of DDX5 and circPHF14 on the migration ability of AGS and 7901 cells. Data are mean ± SD of three independent experiments. * P < 0.05, ** P < 0.01. Scale bar 500μm.