Research Paper Volume 15, Issue 6 pp 2221—2236
Promising antitumor effects of the curcumin analog DMC-BH on colorectal cancer cells
Figure 5. DMC-BH inhibits PI3K-Akt signaling in CRC cells. (A) Western blot analysis of phosphorylated levels of p-PI3K, p-Akt, and p-mTOR after DMC-BH treatment. (B) The phosphorylated levels of p-Akt and p-mTOR measured after incubation with the Akt pathway activator SC79 (10.96 μM). (C) Cell viability analysis of CRC cells treated with DMC-BH and SC79 in combination. (D) Flow cytometry analysis of CRC cells treated with DMC-BH and SC79 in combination. (E) Western blot analysis of the relevant apoptotic proteins (Bcl-2 and c-Casp. 3 and c-PARP) after DMC-BH and SC79 combined treatment.