Research Paper Volume 15, Issue 7 pp 2473—2484

Figure 6. Effects of PRSS2 knockdown on GC cell viability and migratory capacity in vitro. (A, B) PRSS2 expression in MGC-803 and BGC-823 cells transfected with negative control siRNA (si-NC) or siRNAs targeting PRSS2 (si-PRSS2 #1 and #2) for 48h n=3 for each group. (C, D) Cell viability was assessed using a CCK-8 assay in MGC-803 and BGC-823 cells transfected with si-NC or si-PRSS2 #1 and #2 for 48h n=6 for each group. (E, F) Transwell invasion assay was performed to determine the invasion ability of si-PRSS2-transfected MGC-803 and BGC-823 cells for 48h n=3 for each group. (G–I) qPCR detection of invasive pseudopod-related proteins Krp1, WASP-B, and Lasp1. n = 3. ^*P < 0:05, ^**P < 0:01, and ^***P < 0:001 indicate significant difference.