Research Paper Volume 15, Issue 6 pp 1748—1767

Figure 3. FHV-injected cohorts display higher mortality compared to controls throughout the experiment, and mortality of young and aged cohorts is comparable among treatment groups. (A) Bar graphs showing the distribution of individual survival within each ‘Treatment’ group at 24 h, 48 h and 72 h post-treatment in both young and old flies (n = 40 starting in each age-by-treatment group). (B) Survival curves comparing mortality of Tris- and FHV-injected flies recorded during the respirometry experiment (n = 40 flies per experimental condition) and during an independent survival assay carried in parallel (n = 30 flies per experimental condition). A significant difference between 5- and 30-days-old flies is observed following FHV infection (p < 0.0001), but not Tris injection (p = 0.648) in the parallel survival assay, based on a log-rank test. At 3 days post-treatment, no significant difference is observed between young and aged FHV-infected flies in both the respirometry experiment (p = 0.827) and the parallel survival assay (p = 0.562) based on a log-rank test.