Figure 2. Injection of D. melanogaster males with FHV decreases levels of organismal OCR in both young and aged flies, but OCR is also influenced by the time post-treatment in young flies and the time of the day during which measurements are performed. (A–D) Graphs showing pairwise comparisons of variables with significant main effect on OCR: ‘Treatment’ (A), ‘Time Post-Treatment’ (B), and ‘Zeitgeber Time’ (C) and interaction effect: ‘Age * Time Post-Treatment’ (D). OCR measurement values for individual datapoints are plotted. Significant comparisons were determined by post hoc Tukey-Kramer tests within each panel as described in methods. Each symbol represents an individual OCR measurement on a fly.