Research Paper Volume 15, Issue 6 pp 2033—2045

Figure 3. DNA-binding and transcription activation activity of NRF1. (A) EMSA analysis of the binding of NRF1 to RhoA promoter. Proteins were incubated with biotin-labeled probe in the absence or presence of 8- to 2-fold of unlabeled probes for 30 min. (B) DLR assay of NRF1 transcription factor activity in OB. Asterisks indicate statistically significant differences as determined by Student's t-test (^*P < 0.05, ^**P < 0.01).