Research Paper Volume 15, Issue 5 pp 1652—1667

Figure 4. ANGPTL2 promotes VEGF-A-dependent LEC tube formation and migration. (A–E) Associations between ANGPTL2 expression and lymphangiogenic factors (VEGF-A, VEGF-C, FIGF, HGF and FGF2) were analyzed using data from the TCGA database. (F–H) CL1-0 cells were transfected with ANGPTL2 cDNA; CL1-5 and A549 cells were transfected with ANGPTL2 shRNA. Levels of mRNA expression were examined by qPCR. *p < 0.05 compared with controls. (I, J) CL1-0 cells were transfected with ANGPTL2 cDNA. The CM was collected and applied to the LECs with VEGF-A or VEGF-C antibody. LEC tube formation and migration was examined. (K–N) Cells were transfected with ANGPTL2 shRNA. The CM was collected and applied to the LECs with VEGF-A. LEC tube formation and migration was examined. *p < 0.05 compared with CL1-0 CM; #p < 0.05 compared with Control; #p < 0.05 compared with CL1-5 or A549 with ANGPTL2 shRNA CM.