Research Paper Volume 15, Issue 6 pp 1931—1943
Exosomal miR-370-3p increases the permeability of blood-brain barrier in ischemia/reperfusion stroke of brain by targeting MPK1
Figure 5. miR-370-3p directly targets MAPK1. (A) Candidate genes from three databases were shown in a Venn diagram. (B) Luciferase reporter constructs with WT or mutant (MUT) MAPK1 3'-UTR binding sites are shown schematically. (C) Following co-transfection of miR-370-3p mimics with WT or MUT reporter plasmids, luciferase activity in bEND.3 cells was assessed. (D) Interactions between MAPK1 and miR-370-3p were tested using RNA immunoprecipitation. (E, F) Relative MAPK1 expression in bEND.3 cells with miR-370-3p mimics or miR-370-3p inhibitor treatment. (G, H) Levels of MAPK1 in both cerebral I/R rats and OGD/R in bEND.3 cells.