Research Paper Volume 15, Issue 5 pp 1279—1305

Figure 6. SIRT1 regulates IL-6 production in senescent HPDL cells. (A) Expression of SIRT1 after si-SIRT1 or si-control transfection into YPDLs and APDLs. Β-Actin was used as a loading control and the relative protein levels were quantified (^*p < 0.01 vs. control). (B) Expression of IL-6 after si-SIRT1 or control transfection into YPDLs and APDLs. IL-6 production from YPDLs and APDLs was measured by an ELISA (^*p < 0.01 vs. control). (C) Expression of SIRT1 after SIRT1 activator, resveratrol treatment, at the protein level measured by western blotting. Β-Actin was used as a loading control and the relative protein levels were quantified (^*p < 0.01, ^#P < 0.05 vs. none). (D) IL-6 and IL-8 productions from YPDLs and APDLs after resveratrol treatment (0, 50 and 100 μM) (^*p < 0.01 vs. none). Abbreviation: RSV: Resveratrol. Representative data from three experiments are shown.