Research Paper Volume 15, Issue 5 pp 1564—1590

Figure 6. EIF4B and YRDC inhibit the migration and invasion of OS cells in vitro. (A) SiRNA-mediated EIF4B and YRDC knockdown suppressed OS cell proliferation, as determined in the CCK-8 assay. Data represent the mean ± SD (n = 6). (B) EIF4B and YRDC knockdown suppressed cell growth, as determined by the colony formation assay (details are shown in the insets). Error bars represent the mean ± SD of 3 independent experiments. * P < 0.05. (C) HOS and 143B cells were transfected with EIF4B or YRDC siRNA, followed by Annexin V-FITC/PI staining. The percentage of apoptotic cells is shown as the mean ± SD from the 3 independent experiments. * P < 0.05, significantly different compared with the vector group. (D) The effect of EIF4B or YRDC siRNA on cell migration capability was evaluated by a wound-healing assay using HOS and 143B cells. Data are mean ± SD, n = 3. * P < 0.05. Scale bar, 200 μm. (E) EIF4B or YRDC knockdown suppresses cell migration and invasion abilities of HOS and 143B cells, as evaluated by Transwell migration and Matrigel invasion assays. Data represent the mean ± SD (n = 3). * P < 0.05. Scale bar, 200 μm.