Research Paper Volume 15, Issue 4 pp 947—981

Figure 4. Increased glial or neuronal dLdh expression promotes accumulation of neutral lipids in 21 day aged male transgenic fly whole brains. (A) Representative confocal fluorescence microscopy images of neuronal dLdh transgenic male fly brains removed at 21 days of age at 29°C and stained for DNA (Blue = DAPI) and neutral lipids (Red = Nile Red). (B) Quantification of nile red staining mean fluorescence intensity revealed that male flies with neuronal dLdh upregulation have over twice the lipid accumulation as control flies. N = 4–5 (F(2, 10) = 17.69, p = 0.0005). (C) Representative confocal fluorescence microscopy images of glial dLdh transgenic male fly brains dissected out at 21 days of age and stained for DNA (Blue = DAPI) and neutral lipids (Red = Nile Red). (D) Quantification of nile red staining mean fluorescence intensity revealed that male flies with glial dLdh upregulation have over 1.5 times lipid accumulation as control flies. N = 7–8. (F(2, 19) = 19.74, p < 0.0001). Comparisons between genotypes for neuronal and glial dLdh transgenic flies separately were made using one-way ANOVA with Dunnett’s multiple comparisons with control. ^***p < 0.001, ^****p < 0.0001.