Research Paper Volume 15, Issue 4 pp 1052—1073

Figure 1. TIPE2 expression was decreased in human EOC. (A) qRT-PCR was used to measure TIPE2 mRNA expression in clinical samples from EOC (n=12) and non-cancer individuals (n=14). TIPE2 mRNA was shown to be considerably lower in EOC patients than in normal ovarian tissues. (B) Western blot examination of the relative expression of TIPE2 in EOC (n=4) and normal control samples (n=4). TIPE2 expression was normalized to β-actin. (C) qRT-PCR was conducted to assess TIPE2 RNA levels in IOSE80, a non-tumorigenic human-derived ovarian cell line, and 4 EOC cell lines, A2780, OVCAR3, SKOV3, HeyA8 (n=3). β-actin was used as an internal control. Data are presented as mean± SD. Statistical significance is denoted by the following symbols: ***p < 0.001, ****p < 0.0001.