Research Paper Volume 15, Issue 5 pp 1358—1370
Ginkgolide A improves the pleiotropic function and reinforces the neuroprotective effects by mesenchymal stem cell-derived exosomes in 6-OHDA-induced cell model of Parkinson’s disease
Figure 1. Ginkgolide A (GA) promotes WJMSC proliferation and stemness. (A) SH-SY5Y cell viability was determined by MTT assay after exposure to 20, 40, and 80 μM GA for 1, 3, 5, and 7 days. The data represent the mean ± standard deviation. *P < 0.05 vs. 0 μM GA. (B) Cell cycle assessed by flow cytometry after exposure to 80 μM GA. (C) Proliferation and cell cycle-related proteins p-AKT, PCNA, Cyclin E, Cyclin B1, and Cyclin D were examined after GA treatment in WJMSCs by western blot analysis. (D) Western blotting of stemness-related markers OCT4 and Nanog after GA treatment in WJMSCs. (E) Vimentin expression of WJMSC after exposure to 20 μM, 80 μM GA. *P < 0.05, **P < 0.01 vs. ctrl.