Research Paper Volume 15, Issue 7 pp 2450—2459

Figure 3. FKBP11 suppresses the proliferation, invasion and migration of osteosarcoma cells. (A) The knockdown efficiency of FKBP11 in osteosarcoma cells was measured through qRT-PCR and western blotting after the transfection of MG63 and 143B cells with Si-NC and Si-FKBP11, respectively, for 48 h. (B) The proliferative capacity of osteosarcoma was measured by CCK-8 assay. (C) FKBP11 overexpression was detected by western blotting. (D) Results of colony formation assays for osteosarcoma cells. (E) The proliferative capacity of osteosarcoma cells was measured by CCK-8 assay. (F–H) Results of invasion and migration assays of osteosarcoma cells. Scale bars represent 100 μm. (I–J) The protein levels of E-cadherin, N-cadherin, and Vimentin were measured through western blotting analysis. Data are presented as the means ± SDs (n = 3). Comparison of Si-FKBP11 and NC, ^*P < 0.05, ^**P < 0.01, and ^***p < 0.001.