Research Paper Volume 15, Issue 6 pp 1713—1733
Knockout of AMD-associated gene POLDIP2 reduces mitochondrial superoxide in human retinal pigment epithelial cells
Figure 6. POLDIP2 KO reduced mitochondrial superoxide. (A) Analysis of mitochondrial superoxide levels in WT and POLDIP2 KO samples. MitoSox fluorescence is presented as fold change compared to WT control. N-acetyl-L-cysteine (NAC) was used as a positive control. (B) Analysis of mitochondrial membrane potential in WT and POLDIP2 KO samples. TMRM fluorescence is presented as fold change compared to WT control. Carbonyl cyanide 3-chlorophenylhydrazone (CCCP) was used as a positive control. Results are presented as mean ± SEM of 2 biological repeats, each with 3 technical repeats. n.s. not significant, * p<0.05 and *** p<0.001.