Research Paper Volume 15, Issue 6 pp 1713—1733
Knockout of AMD-associated gene POLDIP2 reduces mitochondrial superoxide in human retinal pigment epithelial cells
Figure 1. Generation of POLDIP2 KO cell line. (A) Sanger sequencing showed small indels in the coding sequence of POLDIP2 in the POLDIP2 KO cell line, as indicated by the red arrow. (B) Quantification of indel percentage in knockout cell line compared to WT. (C) RT-qPCR analysis of POLDIP2 repression using CRISPR KO. Values expressed as mean ± SEM, n=3. **** p<0.0001. (D) Western blot analysis of POLDIP2 protein repression.