Figure 1. Summary of transcriptomics data. (A) Experimental Design- samples were taken every hour as indicated from vehicle (Veh) and myriocin (Myr) treated cultures. RNA was isolated and mRNA was quantified using RNA-seq. (B) Differentially expressed genes (DEGs)-RNA-seq data were filtered to include well-annotated genes with sufficient signal intensity prior to statistical analysis. Two-way ANOVA (Time: 6 time points; Drug: two conditions- Veh and Myr) was applied. The ANOVA test produces 3 p-values for each gene, one for the main effect of time (T- black circle), one for the main effect of drug (D- red circle), and one for the ‘Interaction’ between Time and Drug (I- green circle; analyzed separately- see