Research Paper Volume 15, Issue 2 pp 396—420

Figure 1. Constant darkness increases fly lifespan. (A) Removing flies from a standard 12 hr: 12 hr light cycle (LD) and housing in constant darkness (DD) increased fly lifespan in WT Canton-S (CS) male flies (LD n = 197, DD n = 188; P < 0.0001). (B) This affect was robust and replicated in female flies (LD n = 200, DD n = 196; P < 0.0001). (C, D) Light causes significant lifespan shortening in a second laboratory strain w¹¹¹⁸. Both male (C) (LD n = 181, DD n = 171; P < 0.0001) and female (D) (LD n = 186, DD n = 176; P = 0.00017) flies showed lifespan extension when aged under DD as compared to LD conditions.