Research Paper Volume 14, Issue 23 pp 9393—9422
Single nuclei profiling identifies cell specific markers of skeletal muscle aging, frailty, and senescence
Figure 7. Validation of senescent markers in cultured human muscle cells. Quantitative PCR (qPCR) of CDKN1A, MYH8, COL19A1, LRRK2, EDA2R and PDK4 after 7 days of incubation in senescent vs. non-senescent cells. Senescence was induced using Doxorubicin in a cell line of Myogenic Progenitor cells (un-differentiated cells, left) and Myotubes (differentiated HSMMs, right). Expression is shown relative to Actin. 3 replicates in each condition/gene.