Research Paper Volume 14, Issue 23 pp 9730—9757

Figure 6. D-gal increased CD38 expression and decreased Sirt6 expression in myocardial tissues of aging mice, and C3G decreased CD38 expression and increased Sirt6 expression in the tissues. Protein expression was determined using Western blotting (A), and the levels of CD38 (B) and Sirt6 expression (C) were semi-quantitatively analyzed. Levels of the CD38 (D) and Sirt6 (E) mRNAs in mouse heart tissues were detected with real-time PCR. Immunohistochemistry was also used to detect protein expression in mouse myocardial tissues. The expression levels were semi-quantified based on immune signal density and extension. (F–I) show CD38 protein expression, and the expression was semi-quantified (J). (K–N) show Sirt6 protein expression, and the expression was semi-quantified (O). Magnification, 10 ×. ^*P < 0.05, ^**P < 0.01 and ^***P < 0.001.