Figure 4. D-gal induced CD38 expression and decreased Sirt6 expression, whereas C3G decreased CD38 expression and increased Sirt6 expression in H9c2 cells. H9c2 cells were treated with D-gal or C3G, CD38 and Sirt6 protein expression was examined using Western blotting (A), and the levels of CD38 (B) and Sirt6 (C) were analyzed by normalizing β-actin expression. H9c2 cells were treated with OSS-128167 and UBCS039, the protein expression was examined (D), and the levels of CD38 (E) and Sirt6 (F) in the cells were analyzed. H9c2 cells were transfected with CD38 siRNA, protein expression was examined (G), and the levels of CD38 (H) and Sirt6 (I) in the cells were analyzed. H9c2 cells were transfected with Sirt6 siRNA, protein expression was examined (J), and the levels of CD38 (K) and Sirt6 (L) in the cells were analyzed. β-Actin was used as an internal reference. *P < 0.05, **P < 0.01 and ***P < 0.001.