Research Paper Volume 14, Issue 23 pp 9730—9757

Figure 3. CD38 siRNA alleviated the senescence-related index in D-gal-treated H9c2 cells, and Sirt6 siRNA aggravated the senescence-related index. Cell proliferation was detected with the CCK-8 assay (A), and cell apoptosis was detected using flow cytometry (B). β-Gal expression was observed using cytochemistry (C) and semi0quantitatively analyzed (D). Cellular ROS levels were detected with a ROS assay (E), and the NAD+ level (F) and NAD+/NADH ratio (G) were detected with an NAD+/NADH detection kit. Magnification, 10×. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 and ^****P < 0.0001. We repeated this procedure 3 times.