Research Paper Volume 14, Issue 23 pp 9730—9757

Figure 2. Treatment with CD38 siRNA and C3G decreased HK2 mRNA expression and increased Sirt6 and TERT expression in H9c2 cells, and Sirt6 siRNA and D-gal treatment increased HK2 expression and deceased TERT expression. Real-time PCR was used to detect the CD38 mRNA level (A), Sirt6 mRNA level (B), TERT mRNA level (C), and HK2 mRNA level (D) in cells after CD38 siRNA transfection. Real-time PCR was also performed to detect the CD38 mRNA level (E), Sirt6 mRNA level (F), TERT mRNA level (G), and HK2 mRNA level (H) in cells after Sirt6 siRNA transfection. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 and ^****P < 0.0001.