Research Paper Volume 14, Issue 19 pp 8077—8094
Novel small molecular compound 2JY-OBZ4 alleviates AD pathology in cell models via regulating multiple targets
Figure 4. 2JY-OBZ4 reduced Aβ level via regulating the levels of APP secretases in N2a-hAPP cells. (A) ELISA assessment of soluble Aβ42 and Aβ40 in the supernatants of N2a-hAPP cells after treatment with DMSO, 2JY-OBZ4 or Hup-A for 24 h. (B) Western blots and (C–F) quantitative analysis for BACE1, PSEN1, ADAM10 and APP in N2a-hAPP cells. PSEN1 represents catalytic subunit of γ-secretase holoenzyme. MW Molecular weight. n = 3 per group. p value significance is calculated from a one-way ANOVA, data are represented as mean ± SEM. *p < 0.05, compared to controls.