Research Paper Volume 14, Issue 19 pp 7734—7751

Figure 1. Voluntary activity, food intake, core body temperature, and triglyceride-derived fatty acid uptake by brown and white adipose tissue during aging. Young (12 weeks old) and middle-aged (52 weeks old) male (left panels in blue) and female (right panels in red) C57BL/6J mice were single-housed in metabolic home-cages for continuous measurement of (A–D) voluntary locomotor activity (n = 7–8 mice/group), (E–H) food intake (n = 7–8 mice/group), and (I–L) core body temperature with implanted telemetric probes (n = 5 mice/group). Mice were injected with triglyceride-rich lipoprotein-like particles labeled with glycerol tri[³H]oleate and 2-[1-¹⁴C]-deoxyglucose at Zeitgeber Time 12 to assess uptake of the radiolabels by interscapular brown adipose tissue (iBAT) and subscapular BAT (sBAT) (M–P) and by gonadal white adipose tissue (gWAT) and subcutaneous WAT (sWAT) (Q–T) (n = 6–8 mice/group). Bar graphs represent means ± SD. ^*p < 0.05; ^**p < 0.01, according to unpaired t-test or two-way ANOVA and following Šídák's multiple-comparison test.