Figure 5. The collagen deposition of myocardial tissue in each group rats on the 7th day after AMI. To characterize myocardial tissue damage, the formalin-fixed and paraffin-embedded myocardial tissue sections were stained using a masson’s trichrome stain kit; by which collagen is stained blue, nucleus is dyed black; cytoplasma and muscle fibers is red. Stained sections were observed under optical microscopy, and were photographed by digital camera. (A–D) Photos of myocardial sections stained by masson G in rats of the Ctrl, HL, AMI, and HL-AMI group, respectively (×100).