Research Paper Volume 14, Issue 18 pp 7527—7546

Figure 5. UUO-induced kidney fibrosis was suppressed in FAT10-deficient mice. (A and B) Representative HP and FAT10 staining in the kidneys from FAT10^+/+ (A) and FAT10^−/− (B) mice subjected to either UUO or sham operation; scale bar = 50 μm. (C and D) Representative Masson’s trichrome (C) and picrosirius red staining (D) of kidney sections from FAT10^+/+ and FAT10^−/− mice with or without UUO for 7 days. (E and F) Immunohistochemistry of protein expression of α-SMA (E) and Collagen IV (F) in obstructed kidneys from FAT10^+/+ and FAT10^−/− mice subjected to either UUO or sham operation. (G and H) Bar graph (right) shows quantification of fibrotic areas in histological sections; ^**P < 0.01 versus FAT10^+/+ mice at the same time point; n = 6. (I and J) Bar graph shows quantification of areas of positive cells; ^**P < 0.01 versus FAT10^+/+ mice at the same time point; n = 6.