Research Paper Volume 14, Issue 18 pp 7527—7546

Figure 1. Hypoxia-induced CHK1 upregulation in RTECs is depend on FAT10. (A) Upon hypoxia treatment for 24 h, mass spectroscopic analysis was performed to detect protein expression in RTECs from FAT10^+/+ mice (n = 3) and FAT10^−/− mice (n = 3). (B) Determination (left) and quantification (right) of the FAT10 and CHK1 protein levels in FAT10^+/+ RTECs or FAT10^−/− RTECs after hypoxia injury. Tubulin was used as a loading control. ^*P < 0.05, ^**P < 0.01. (C) Determination (left) and quantification (right) of the FAT10 and CHK1 protein levels in HK-2 cells following treatment with hypoxia or without hypoxia. ^*P < 0.05, ^**P < 0.01. (D) The protein and mRNA levels of FAT10 and CHK1 in HK-2 cells following treatment with hypoxia or without hypoxia. ^**P < 0.01. (E) Determination (left) and quantification (right) of the FAT10 and CHK1 protein levels in HK-2 cells transfected with shFAT10 following hypoxic injury. ^**P < 0.01. (F) Upon hypoxia treatment for 24 h, the mRNA levels of FAT10 and CHK1 in HK-2 cells transfected with shFAT10. ^**P < 0.01.