Research Paper Volume 14, Issue 18 pp 7505—7516

Figure 1. ZCCHC12 was upregulated in OS. (A) Differences in gene expression between OS and normal bone tissues represented by a volcano plot. (B) KEGG enrichment analysis indicated that PI3K/AKT signaling pathways had been significantly altered. (C) ZCCHC12 expression was detected in OS cell lines by qRT-PCR. (D) Heatmap of differential gene expression in OS and normal bone tissues. n = 5; ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001.