Research Paper Volume 14, Issue 17 pp 7137—7155

Identification of alanine aminotransferase 1 interaction network via iTRAQ-based proteomics in alternating migration, invasion, proliferation and apoptosis of HepG2 cells

class="figure-viewer-img"

Figure 8. (A) RT-qPCR measured the relative mRNA expression levels of LAMB3, KDM6A, ASPP2, ANXA2, PDIA6, HSPB1, KI67, and EP-CAM. (B) A representative Western blot analysis for ASPP2, LAMB3, HSPB1, KI67, and EP-CAM expression in HepG2 cells. (C) A representative Western blot analysis for EP-CAM, KI67, and ASPP2 co-immunoprecipitation with ALT1 used as a bait protein. (D) A representative Western blot analysis for ALT1 expression in EP-CAM, KI67, and ASPP2 knockdown HepG2 cells.