Research Paper Volume 14, Issue 17 pp 7052—7064
Bruton tyrosine kinase (BTK) may be a potential therapeutic target for interstitial cystitis/bladder pain syndrome
Figure 5. In vitro verification of the effect of BTK on the proliferation and metastasis of HMC-1 cells. (A) Western blotting displayed the knockdown efficiency of sh-BTK. (B) HMC-1 cell proliferation was detected by CCK-8 kit. (C) Wound-Healing assay showed the migration ability of HMC-1 cells. Scale bar: 100 μm. (D) Transwell assay was used to detect HCC-1 cell invasion. Scale bar: 100 μm. (E–G) Quantifications of mast cell mediators, including (E) Tryptase, (F) β-hexosaminidase and (G) Histamine. In all cases, Values are mean ± SD (n=3 for each group; *P<0.05, **P<0.01).