Research Paper Volume 14, Issue 17 pp 6887—6904
Fetal programming: in utero exposure to acrylamide leads to intergenerational disrupted ovarian function and accelerated ovarian aging
Figure 6. (I) TUNEL staining (red) of ovaries from AF1 females and normal CF2 females (A–C), and ovaries from AF1 with the dose of 2.5 mg/kg (D–F), 10 mg/kg (G–I), and 20 mg/kg (J–L). Both granulosa cells and oocyte nuclei were stained with Hoechst (blue). (II) TMR relative intensity in 4-week-old AF1 females compared with control CF1. (III) TUNEL staining (red) of ovaries from AF2 females and normal CF2 females (A–C), and ovaries from AF1 with the dose of 2.5 mg/kg (D–F), 10 mg/kg (G–I), and 20 mg/kg (J–L). Both granulosa cells and oocyte nuclei were stained with Hoechst (blue). (IV) TMR relative intensity in 4-week-old AF2 females compared with control CF2. Scale bar = 60μm.