Research Paper Volume 14, Issue 17 pp 6887—6904
Fetal programming: in utero exposure to acrylamide leads to intergenerational disrupted ovarian function and accelerated ovarian aging
Figure 4. (I) Effect of developmental ACR exposure on immunolocalization of ovarian CYP19 localization in AF1 treated groups compared to control CF1. Normal control CF1 females (A–C). Immunolocalization of CYP19 protein in AF1 females treated with the dose of 2.5 mg/kg (D–F), 10 mg/kg (G–I), and 20 mg/kg (J–L). (II) CYP19 relative intensity in 4-week-old AF1 females compared with control CF1. (III) Effect of developmental ACR exposure on immunolocalization of ovarian CYP19 localization in AF2 treated groups compared to control CF2. The normal control CF2 females (A–C), immunolocalization of CYP19 protein in AF2 females treated with the dose of 2.5 mg/kg (D–F), 10 mg/kg (G–I)), and 20 mg/kg (J–L). (IV) The CYP19 relative intensity in 4-week-old AF2 females compared with control (CF2). Sections of the ovary was performed by immunofluorescence using specific CYP19 antibody stained with FITC (green), and cell nuclei were stained with Hoechst (blue). Scale bar = 20 μm.