Research Paper Volume 14, Issue 17 pp 7003—7013
UBE2C mediated radiotherapy resistance of head and neck squamous cell carcinoma by regulating oxidative-stress-relative apoptosis
Figure 2. The knocking-down of UBE2C sensitized CAL27 cell to radiation in vitro. (A) RT-PCR results of UBE2C gene expression in CAL27 after Si-UBE/Con Lentivirus transfection. (B) Western blot results of UBE2C protein expression in CAL27 after Si-UBE/Con Lentivirus transfection. (C) Transwell (without matrigel) results of Si-UBE/Con CAL27 cells under 0/2 Gy radiation after 24h. (D) Transwell (with matrigel) results of Si-UBE/Con CAL27 cells under 0/2 Gy radiation after 24h. (E) Wound healing results of Si-UBE/Con CAL27 cells under 0/2 Gy radiation after 48h. (F) Clone formation results of Si-UBE/Con CAL27 cells under 0/2 Gy radiation after 14d. *P < 0.05, **P < 0.01, ***P < 0.001 versus the control.