Senescent phenotype of astrocytes leads to microglia activation and neuronal death

Wenyou Zhang; Xuehan Yang; Jingyue Liu; Yichen Pan; Ming Zhang; Li Chen

doi:doi:10.18632/aging.

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Research Paper

Senescent phenotype of astrocytes leads to microglia activation and neuronal death

Figure 6. The indirect effect of aged astrocytes in communicating with microglia and neurons. (A) A schematic diagram of the interaction of cell supernatants. (B) Cell viability assay of BV2 cells by using astrocytes treated with or without H₂O₂ supernatant. (C) Cytokine ELISA of IL-6 in culture medium of BV2 treated with astrocyte supernatant (treated with or without H₂O₂) expressed as pg cytokine per mL of culture medium without serum. (D) Cell viability assay of N2a cells by using BV2 treated with astrocytes supernatant. (E) The caspase-3 activity was measured with a caspase-3 assay kit. All experiments were expressed as the mean +/−S.D, analyzed by ANOVA followed by Tukey’s test, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ns represents no significance.